Essay about My Personal Experience in Research Projects

Research Experience I have proactively engaged in research activity throughout my undergraduate program. I had my first research experience in Dr. B Anand’s lab, Indian Institute of Technology (IIT), Guwahati, during my second year. The aim of the project was to study the non-fluorescent beta-barrel structured proteins and engineer its amino acid sequence to make them into fluorescent ones. Our approach was mainly based upon the studies of Green Fluorescent Protein (GFP) from a jellyfish Aequorea victoria. Tools like Python programming language, Gromacs, Ribosome, PROSS, and Pymol were used to study the structural components of non-fluorescent proteins. I gained invaluable†¦show more content†¦I was challenged with cloning ydeH gene into the pJT118 plasmids which are green light sensitive. The ydeH gene produces a protein named diguanylate cyclase, which induces the production of c-di-GMP in Rosetta strain. This project gave me an opportunity to participate and win silver at Internationa l Bio-molecular design competition (BIOMOD) 2012 held at Wyss Institute, Harvard University, USA. Most of the molecular genetic techniques were extensively used in my project. During this internship, apart from gaining deeper technical experience, I realized that I could work on a research problem with a certain degree of independence, figuring out right approaches and proposing useful experiments. I acquired more confidence in my aptitude for research and it has been boosted while working on my undergraduate projects. As a part of my undergraduate thesis, I worked on a project ‘Extraction and analysis of beauvericin, a mycotoxin produced by Beauveria bassiana and its cytotoxic effects on the SF-21 (immortalized pupal ovarian cells isolated from Spodoptera frugiperda) cell line’ under the guidance of Dr. Gurvinder Kaur Saini, IIT Guwahati. Beauvericin metabolite was extracted from the Beauveria bassiana isolates UB17 and UB21, by using n-hexane extraction method and the presence of beauvericin was analyzed and confirmed with High Pressure Liquid Chromatography (HPLC) and Mass spectroscopy (MS). CytotoxicShow MoreRelatedData Catalog For Enterprise Geodatabase1008 Words   |  5 PagesSpatial information is usable when it has metadata, as it is straightforward to follow and find datasets. GIS data catalog for Enterprise Geodatabase is a project which allows acquiring information regarding the availability of GIS datasets and related properties such as quality, description, the point of contact, extent, etc. The audience for the project are the internal customers looking for data. 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